Jackson who is standing down from the European Parliament and is rapporteur for the revision of the Waste Framework Directive, made the payment as consultancy fees, according to the Sun newspaper.
The Sun asked for details after the MP expenses scandal.
Jackson issued a statement on the matter saying: Over 25 years I have employed a regular staff consisting of a secretary and a press agent in the UK and PA in Brussels. None of these has been a family member. I have a special interest as a member of the PE Environment Committee in questions of waste management policy. I published a pamphlet on this subject in 2006. In this connection my husband who has experience in this field and was for 14 years a consultant to a French engineering company active in this business in the UK and across Europe, provided advice and assistance. I paid him an appropriate professional fee.
The newspaper reported that the total figure was initially not made public when she listed the money given to her husband Robert, splitting it into three and listing each part as between £0 and £10,000.
The Sun also claimed that Jackson was also paid £6,000 a year as an adviser to waste management firm Shanks.