The Waste Scotland Regulations 2005 this week placed a duty of care on residents not to transfer their rubbish to unauthorised parties.
The Scottish Executive said evidence from councils showed a large percentage of waste fly-tipped in Scotland was from households.
Environment Minister Ross Finnie said: "These measures strengthen the protection that existing waste regulations provide. By tightening regulations on household waste we will further reduce fly-tipping in Scotland."
The legislation also ended the rights of quarries, mines and agricultural sites to opt out of Scottish waste laws.
Finnie added: "Bringing wastes from farming, mining and quarrying under full control will bring greater protection to the environment and human health."