The Scottish Environment Protection Agency (Sepa) will no longer allow unlicensed storage of waste tyres.
Under rules that took effect on 30 March, all operators who store or process waste tyres will be required to hold a licence under the Waste Management Licensing (Scotland) Amendment Regulations 2016.
Operators had previously been permitted to process 10 tonnes of tyres a week and store up to 1,000 waste tyres on a secure site for up to a year if they registered an exemption with Sepa and the storage was with a view to recovery or reuse.
They now have until 30 September to either apply for a waste management licence or cease operations.
Those who continue unlicensed after that date face a fine of up to £40,000 and/or six months imprisonment.
Sepa executive director Calum MacDonald said: “Illegitimate waste tyre operators undercut compliant companies by flouting the regulations, and putting the environment and communities at risk.
“The new strengthened regulations will be a key asset in helping us to combat illegal waste tyre sites, and hold those responsible to account.”